Author/Editor		Mikek, Kristina; Hawlina, Marko; Drnovšek-Olup, Brigita; Pfeifer, Vladimir
Title		Konfokalna mikroskopija roženice
Translated title		Confocal microscopy of the cornea
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 73, št. 5
Publication year		2004
Volume		str. 445-50
Language		slo
Abstract		Background. Confocal microscopy is a diagnostic method used for examination of the morphological structure of the cornea in vitro as well as in vivo. Methods. Using a confocal microscope (Confoscan 2.0, Nidek, Japan), we can determine and analyze morphological changes in individual layers of the cornea, from epithelial to stromal and endothelial ones in addition, it is possible to observe keratitis causing agents. According to the findings revealed, we choose a treatment method and a surgical procedure type. The investigation is aimed to determine a cellular density in different layers of the corrcea, i. e., that of epithelial cells, stromalkeratocytes and endothelial cells. Monitoring cellular density by layers helps us understand the cell response after corneal damage or surgery of the anterior segment of the eye. Conclusions. The method is clinically applicable also in an opaque cornea occurring as a result of epithelial or stromal edema. Such investigations were not feasible before the introduction of the confocal microscopy.
Summary		Izhodišča. Konfokalna mikroskopija (KM) je preiskovalna metoda, s katero pregledujemo morfološko strukturo roženice in vitro in in vivo. Metode. S konfokalnim mikroskopom (Confoscan 2,0, Nidek, Japonska) opazujemo in analiziramo morfološke spremembe v posameznih plasteh roženice: epitelne, preko strome do endotelne plasti. Opazujemo lahko povzročite ja roženičnega vnetja in se odločamo o načinu zdravljenja ter o vrsti kirurškega posega. Raziskujemo gostoto celic v posameznih plasteh roženice: epitelnih celic, keratocitov v stromi ter endotelnih celic. Z opazovanjem gostote celic v posameznih plasteh roženice ugotavljamo celični odgovor pri poškodbah, vnetjih roženice ali po operacijah sprednjega segmenta očesa. Zaključki. S KM opazujemo tudi neprozorno roženico, ki nastane zaradi edema epitelne plasti ali strome, česar in vivo pred odkritjem KM ni bilo mogoče.
Descriptors		CORNEAL DISEASES MICROSCOPY, CONFOCAL CORNEA KERATITIS KERATOCONUS CORNEAL DYSTROPHIES, HEREDITARY