| Author/Editor | Šmid, Lojze M; Zupan, Jure | |
| Title | Morfološka analiza presadkov stromalnih matičnih celic kostnega mozga v možganih podgane | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2003 | |
| Volume | str. 54 | |
| Language | slo | |
| Abstract | BACKGROUND. Stem cells have a great therapeutic potential for future replacement of diseased or damaged tissue, due to their ability to differentiate into different cell lines. Beside embryonic it is known that there are also adult stem cells, which include bone marrow stromal cells (BMSC). The therapeutic potential of BMSC is due to their characteristics: they are easily accessible, easily grown in vitro, their low immunogenicity allows autologous transplantation without a danger of rejection potential, and they can differentiate into different cell types. AIM. The aim of our study was to evaluate (1) the survival of grafted human BMSC, their (2) distribution and (3) differentiation into neural tissue cell types 28 days after grafting into rat striatum. In the study we compared the outcome of BMSC grafting into the previously inflamed brain parenchyma to their grafting into the non-inflamed brains of the control group. HYPOTHESES. I. Human BMSC grafted into the rat striatum survive the observation period, they migrate through the recipient brain and differentiate into different neural tissue cell types. II. Inflammation at the site of graft placement enhances survival, migration and differentiation of grafted BMSC. METHODS. Female albino Wistar rats were divided into 5 groups (n,.s=6): A - BMSC grafted into inflamed striatum, B - BMSC grafted into intact striatum, C - striatal inflammation without a graft, Č - double labeled BMSC graft, D - dead BMSC graft . The rats were euthanized 28 days post-grafting in deep anesthesia, their brains were removed, cut into 20 pm thick coronal sections, and processed with imunohistochemical (IHC) and imunofluorescent (IF) methods to reveal various neural cell antigens.. Using a microscope we analyzed survival of grafted BMSC, their migration and differentiation. (Abstract truncated at 2000 characters). | |
| Descriptors | BONE MARROW TRANSPLANTATION STROMAL CELLS CORPUS STRIATUM GRAFT SURVIVAL IMMUNOHISTOCHEMISTRY FLOW CYTOMETRY FLUORESCENT ANTIBODY TECHNIQUE RATS, WISTAR |