Author/Editor     Žele, Tilen
Title     Razlike med spoloma v regeneraciji senzoričnih aksonov po poškodbi perifernega živca pri podgani
Type     monografija
Place     Ljubljana
Publisher     Medicinska fakulteta
Publication year     2003
Volume     str. 44
Language     slo
Abstract     BACKGROUND. After peripheral nerve injury, motor axon regeneration and functional recovery is faster in female than in male rats. Possible sex related differences in the regeneration of sensory axons have not been examined yet. AIM. To study possible sex related differences in the rate of early regeneration of sensory axons and recovery of nociception after the peripheral nerve injury in the rat. HYPOTHESES. We hypothesized that early regeneration of sensory axons after peripheral nerve crush is faster in female than in male rats, and that the recovery of nociception after such an injury is faster and more extensive in females than in males. If so, we further hypothesized that the faster regeneration of sensory axons in female rats is not due to the effects of the circulating ovarian hormones but, rather, is related to the more effective cell support for the axons in the nerve segment distal to the injury. METHODS. In the first experiment, the sural nerve was crushed in normal female and male rats, and ovariectomised female rats. The regeneration of sensory axons was followed during 8 days after the nerve injury. The elongation of the fastest regenerating nociceptive axons was assessed by the nerve pinch test. The abundance of regenerating axons was examined in nerve cross sections 8 mm distal from the crush site by the immunohistochemical reaction to neurofilament. In the second experiment, the sural nerve was crushed in intact male and female rats, and the adjacent nerves were transected and ligated. Then recovery of the nociception in the skin of the instep of the right foot was monitored for 24 weeks by the skin pinch test. In the third experiment, the sural nerve was crushed and its segment just distal to the crush lesion was made acellular by freezing and thawing in intact female and male rats, and ovariectomised female rats. (Abstract truncated at 2000 characters).
Descriptors     SURAL NERVE
NERVE CRUSH
NERVE REGENERATION
AXONS
PAIN THRESHOLD
RATS, WISTAR
AXONS
OVARIECTOMY
IMMUNOHISTOCHEMISTRY
NEUROFILAMENT PROTEINS
ESTRADIOL
TIME FACTORS
SEX FACTORS