Author/Editor		Hajdinjak, Tine; Zagradišnik, Boris
Title		Prostate cancer and polymorphism D85Y in gene for dihydrotestosterone degrading enzyme UGT2BI5: frequency of DD homozygotes increases with Gleason score
Type		članek
Source		Prostate
Vol. and No.		Letnik 59, št. 4
Publication year		2004
Volume		str. 436-9
Language		eng
Abstract		BACKGROUND. Although, a functional rationale for influence of polymorphism D85Y in gene UGT2B15 on prostate cancer (PCa) exists (different Vmax of enzyme), conflicting results have been reported. METHODS. DNA from 178 controls and 206 PCa patients with known Gleason score were genotyped using a newly developed RFLP assay, which allowed the detection of both alleles in an individual after single PCR amplification. RESULTS. Controls:16% DD, 52% DY; PCa patients: 23% DD, 49% DY. Subgroups of PCa: well differentiated: 11% DD, 37% DY; moderately differentiated: 22% DD, 50% DY; poorly differentiated: 34% DD, 50% DY. Correlation was confirmed between Gleason score and number of D alleles (P = 0.018) and persisted after age adjustment. When comparing controls to patients with a Gleason score of 7 or more, difference for the frequency of homozygosity DD was significant between the groups (P = 0.032, OR = 2.04). CONCLUSIONS. Polymorphism D85Y in gene UGT2B15 correlates with differentiation of PCa.
Descriptors		PROSTATIC NEOPLASMS POLYMORPHISM (GENETICS) GLUCURONOSYLTRANSFERASE GENOTYPE POLYMERASE CHAIN REACTION CASE-CONTROL STUDIES