Author/Editor		Hajdinjak, Tine; Zagradišnik, Boris; Kokalj-Vokač, Nadja; Kisner, Karel
Title		Genetski dejavniki in rak prostate (vpliv polimorfizmov C825T v genu GNB3 in D85Y v genu UGT2B15)
Translated title		Genetic factors and prostate cancer (influenece of single nucleotide polymorphisms C825T in gene GNB3 and D85Y in gene UGT2B15)
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 73, št. 6
Publication year		2004
Volume		str. 481-4
Language		slo
Abstract		Background. In Slovenia, incidence of prostate cancer (CaP) is approximately half of incidence in neighbouring Austria. With ageing of population and increasing awareness, incidence is increasing. Treatment of CaP can drastically decrease quality of life and course of disease is not possible to predict. As main risk factor for CaP is heredity, one way of looking for prognostic factors is evaluation of influence of single nucleotide polymorphisms (SNPs). Methods. DNA was isolated from peripheral blood of 86 patients with histologically proven prostate cancer and 178 controls. Polymorphisms D85Y in gene UGT2B15 and C825T in gene GNB3 were evaluated with RFLP. Frequencies of alleles were compared between controls and patients and between patients stratified according to the Gleason score (less than 7 and 7 or more). Results. D85Y: patients: 23% DD, 49% DY, controls 16% DD, 52% DY (NS). In patients with Gleason score 7 or more frequency of DD was 33% and with Gleason score less than 7-15% (risk ratio for DD 2.97, p = 0. 041). C825T: patients: 14% TT, 44% CT, controls 8% TT, 46% CT (NS). Conclusions. Although study did not confirm influence of evaluated polymorphisms on risk for developing prostate cancer, we identified significantly higher frequency of D allele of polymorphism D85Y in subgroup of patients with poorly differentiated CaP. This polymorphism could become one of the prognostic factors in CaP.
Summary		Izhodišča. V Sloveniji odkrijemo približno polovico manj raka prostate (CaP) kot v Avstriji. S staranjem prebivalstva in zlasti širjenjem osveščenosti se incidenca veča. Načini zdravljenja lahko močno poslabšajo kakovost življenja. Poteka bolezni ni moč natančno napovedati. Glavni dejavnik tveganja za CaP je dednost, zato je eden od načinov iskanja novih napovednih dejavnikov analiza polimorfizmov posameznih nukleotidov (SNP). Metode. DNK smo osamili iz periferne krvi 86 bolnikov s histološko verificiranim rakom prostate in 178 kontrol Polimorfizma DBSY v genu UGT2B75 in C825T v genu GNB3 smo določali z metodo RFLP. Frekvence posameznih alelov smo primerjali med bolniki in zdravimi ter znotraj skupine bolnih glede na oceno po Gleasonu (pod 7 in 7 ali več). Rezultati. D85Y. bolniki; 23% DD, 49% DY, kontrolie: 16% DD, 52% DY (nz). Med bolniki z oceno po Gleasonu 7 ali več je bil delež DD 33%, pod 7 pa 14% (tveganje za DD 2,97, p = 0, 047). C825T; bolniki: 14% TT; 44% CT, kontrole 8% TT, 46% CT(nz). Zaključki. Vpliva preučevanih polimorfizmov na tveganje za nastanek CaP nismo potrdili, ugotovili pa smo značilno večji delež alela D polimorfizma D85Y znotraj podskupine bolnikov s slabše diferenciranim rakom. Ta bi lahko postal eden od napovednih dejavnikov pri odkrivanju in zdravljenju CaP.
Descriptors		PROSTATIC NEOPLASMS POLYMORPHISM (GENETICS) GENOTYPE POLYMORPHISM, RESTRICTION FRAGMENT LENGTH