| Author/Editor | Ambrožič, Aleš | |
| Title | Lastnosti in mehanizmi trombogenega delovanja protiteles proti beta2-glikoproteinu 1 | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 2004 | |
| Volume | str. 98 | |
| Language | slo | |
| Abstract | The in vivo physiologic and pathogenic role of beta2-glycoprotein I (beta2GPI), a 50 kD plasma protein, is presumed to be related to its interactions with negatively charged phospholipid membranes interfering also the coagulation system. beta2GPI appears to be the major antigen for antiphospholipid antibodies (aPL). Antibodies against beta2GPI (anti-beta2GPI) are signiffcantly associated with thrombotic events. Mechanisms of pathogenic action of antiBZGPI are not completely elucidated yet, neither is known if subsets of anti-beta2GPI differ in their pathogenic action. The main aim of our work was to identify possible pathogenic mechanisms of anti-beta2GPI in patients with antiphospholipid syndrome (APS). At the beginning we estimated the ELISA method for detection of anti-beta2GPI. Other objectives of our work were to find out a group of patients with anti-beta2GPI, but without clinical signs of APS, and to compare properties of anti-beta2GPI from this group with anti-beta2GPI from patients with APS. A high frequency (42%) of IgG anti-beta2GPI was detected in children with atopic dermatitis (AD), but not in those with other allergic diseases. The epitopes were identified in domain V of BZGPI and the antibody binding was abolished upon the specific proteolytic cleavage of the phospholipid binding C-terminal loop in domain V of beta2GPI. The epitopic difference from anti-BZGPI in APS may explain presumed non-thrombogenicity of anti-beta2GPI in children with AD. Our results point to substantial differences in the binding to beta2GPI coated on different microtiter plates by anti-beta2GPI in AD but not by anti-beta2GPl in APS. In contrast to the other plate types. (Abstract truncated at 2000 characters) | |
| Descriptors | ANTIBODIES, ANTIPHOSPHOLIPID GLYCOPROTEINS THROMBOSIS ANTIPHOSPHOLIPID SYNDROME IGG AUTOIMMUNE DISEASES DERMATITIS, ATOPIC |