Author/Editor		Žolnir-Dovč, Marija
Title		Molekularno epidemiološka raziskava izolatov bakterije Mycobacterium tuberculosis v Sloveniji
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2004
Volume		str. 95
Language		slo
Abstract		The most important factors responsible for the successful control of tuberculosis (TB) are rapid detection of TB patients, administration of adequate and efficient therapy and implementation of good contact-tracing procedures to stop further transmission. However, in the recent years DNA fingerprinting of Mycobacterium tuberculosis has given new insight into the nature of TB transmission. In a prospective nation-wide, oneyear DNA fingerprinting study, the genetic diversity of 99.7% of all M tuberculosis isolates obtained from Slovenian patients with culture-verified TB in 2001 were assessed using a standardized IS6110 restriction fragment length polymorphism (RFLP) method. To the best of our knowledge, our study represents the first nation-wide study which was performed in a country with a moderate TB incidence. Among 306 M tuberculosis isolates analysed, 228 different IS6110 RFLP patterns were found. The number of IS6110 copies varied from 2 to 16 (9.2 copies per isolate on average). Only 2 isolates (0.7%) with less than five IS6110 copies were identified. Clustered M tuberculosis isolates were detected in 116 (37.9%) patients. The degree of recent transmission in one year period was 25%. (Abstract truncated at 2000 characters)
Descriptors		MYCOBACTERIUM TUBERCULOSIS TUBERCULOSIS GENOTYPE EPIDEMIOLOGY, MOLECULAR RISK FACTORS POLYMORPHISM, RESTRICTION FRAGMENT LENGTH POLYMERASE CHAIN REACTION DIAGNOSTIC ERRORS PROSPECTIVE STUDIES SLOVENIA