Author/Editor		Lončar, Darja
Title		Virulenčni dejavniki sevov bakterije Escherichia coli izolirani iz urina bolnikov z infekcijo sečil
Translated title		Virulence determinants of Escherichia coli strains isolated from urine of patients with urinary tract infection
Type		monografija
Place		Ljubljana
Publisher		Biotehniška fakulteta, Enota medoddelčnega študija mikrobiologije
Publication year		2002
Volume		str. 56
Language		slo
Abstract		Urinary tract infections are the most commom bacterial infections and are most frequent among women.. Infections are rare in men and are in most cases complicated. Escherichia coli is the most common cause of urinary tract infections, as it can be transmitted from the gastrointestinal tract to the urinary tract. Bacteria first infect the urethra (urethritis) and subsequently bladder (cystitis), the kidney (pyelonefritis) and prostata (prostatitis) in men. A hallmark of virulent E.coli strains is their possession of virulence factors, traits that confer pathogenic potential and are infrequent among commensal strains as well as pathogenicity islands, bloks of virulence factors genes. We based our researsh on following virulence factors: P-fimbriae, S-fimbriae, afimbrial adhesin, capsular polysaccharid coat, alpha-hemolysin, cytotoxic necrotizing factor type I, aerobactin and production of colicin. We have examined the presence of these virulence factors and their distribution in relation to sex and age of patients. Our results showed that UTI was most frequent in women between the age of 15 and 65 years and in men younger than 15 years. A significiant difference was between virulence factors isolated from women or from men. Isolates from men showed a higher frequency of hemolytic strains and strains producing CNF1. CNF1, alpha-hemolysin, P- and S- fimbriae were often present together, indicating that they may be encoded by pathogenicity islands.
Descriptors		URINARY TRACT INFECTIONS ESCHERICHIA COLI VIRULENCE FIMBRIAE, BACTERIAL ADHESINS, ESCHERICHIA COLI BACTERIAL CAPSULES HEMOLYSINS CYTOTOXINS COLICINS POLYMERASE CHAIN REACTION ELECTROPHORESIS, AGAR GEL