Author/Editor		Križan-Hergouth, Veronika
Title		Epidemiološka genotipizacija proti meticilinu odporne bakterije Staphylococcus aureus (MRSA) izolirane iz krvi bolnikov v sepso s Sloveniji
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2004
Volume		str. 98
Language		slo
Abstract		Staphylococcus (S.) aureus is one of the most frequent etiologic agents of sepsis and various other infections. In the last years the number of methicillin-resistant isolates MRSA has increased. Today MRSA is one of the most important causes of hospital infections world-wide, less often it is found in the community. The prevalence of MRSA isolates differs among geographic areas, but particularly in the south Europe, America and Asia it exceeds 30 %. By typing it was established that some MRSA strains spread rapidly and more successfully than others inside the hospitals and among them in wide geographic areas. Many epidemic strains, which predominate in particular country and also some international epidemic strains, have been described. Until now no data about MRSA genotypes and their distribution in Slovene hospitals were available. In our study we wanted to determine the number of different genotypes of MRSA blood culture isolates in Slovenia in the years 2000 and 2001 and whether an epidemic strain was present in different hospitals. We also wanted to determine the number of patients with MRSA sepsis in Slovene hospitals in the years 2000 and 2001 and the proportion of MRSA in S aureus blood culture isolates. We also wanted to describe the characteristics of patients with MRSA sepsis, to confirm resistance to methicillin by mecA gene detection and to determine the susceptibility to other antibiotics. Typing of MRSA isolates was performed by pulsed-field gel electrophoresis - PFGE, which is the reference typing method for staphylococci. Chromosomal DNA was digested by rare cutting restriction enzyme Snzal. DNA fragments were resolved by PFGE. The isolates were grouped according to their patterns to different genotypes if they differed in more than three fragments. The patient's data were obtained from medical doctors in hospitals and microbiologists in laboratories, who sent us MRSA isolates. (Abstract truncated at 2000 characters)
Descriptors		SEPSIS STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE GENOTYPE CROSS INFECTION HOSPITALS DNA ELECTROPHORESIS, GEL, PULSED-FIELD POLYMERASE CHAIN REACTION