Author/Editor		Sepčić, Kristina; Berne, Sabina; Rebolj, Katja; Batista, Urška; Plemenitaš, Ana; Šentjurc, Marjeta; Maček, Peter
Title		Ostreolysin, a pore-forming protein from the oyster mushroom, interacts specifically with membrane cholesterol-rich lipid domains
Type		članek
Source		FEBS Lett
Vol. and No.		Letnik 575
Publication year		2004
Volume		str. 81-5
Language		eng
Abstract		Ostreolysin, a 15 kDa pore-forming protein from the edible oyster mushroom (Pleurotus ostreatus), is lytic to membranes containing both cholesterol and sphingomyelin. Its cytotoxicity to Chinese hamster ovary cells correlates with their cholesterol contents and with the occurrence of ostreolysin in the cells detergent resistant membranes. Moreover, ostreolysin binds to supported monolayers and efficiently permeabilizes sonicated lipid vesicles, only if cholesterol is combined with either sphingomyelin or dipalmitoylphosphatidylcholine. Addition of mono- or di-unsaturated phosphatidylcholine to the cholesterol/ sphingomyelin vesicles dramatically reduces the ostreolysin's activity. It appears that the protein recognizes specifically a cholesterol-rich lipid phase, probably the liquid-ordered phase.
Descriptors		PORINS AGARICALES MYCOTOXINS CHOLESTEROL SPHINGOMYELINS 1,2-DIPALMITOYLPHOSPHATIDYLCHOLINE CHO CELLS ELECTRON SPIN RESONANCE SPECTROSCOPY CELL MEMBRANE PERMEABILITY