Author/Editor		Kostanjšek, R; Lapanje, A; Rupnik, M; Štrus, J; Drobne, D; Avguštin, G
Title		Anaerobic bacteria in the gut of terrestrial isopod Crustacean Porcellio scaber
Type		članek
Source		Folia Microbiol (Praha)
Vol. and No.		Letnik 49, št. 2
Publication year		2004
Volume		str. 179-82
Language		eng
Abstract		Anaerobic bacteria from Porcellio scaber hindgut were identified and, subsequently, isolated using molecular approach. Phylogenetic affiliation of bacteria associated with the hindgut wall was detennined by analysis of bacterial 16S rRNA gene sequences which were retrieved directly from washed hindguts of P. scaber. Sequences from bacteria related to obligate anaerobic bacteria from genera Bacteroides and Enterococcus were retrieved, as well as sequences from 'A 1 subcluster' of the wall-less mollicutes. Bacteria from the genus Desulfotomaculum were isolated from gut wall and cultivated under anaerobic conditions. In contrast to previous reports which suggested the absence of anaerobic bacteria in the isopod digestive system due to short retention time of the food in the tube-like hindgut, frequent renewal of the gut cuticle during the moulting process, and unsuccessful attempts to isolate anaerobic bacteria from this environment our results indicate the presence of resident anaerobic bacteria in the gut of P. scaber, in spite of apparently unsuitable, i.e. predominately oxic, conditions.
Descriptors		DIGESTIVE SYSTEM BACTERIA, ANAEROBIC CRUSTACEA PHYLOGENY