| Author/Editor | Gspan, Mateja; Vrtačnik, Margareta; Ambrožič-Dolinšek, Jana; Kovač, Maja; Camloh, Marjana; Žel, Jana | |
| Title | Tissue culture of pyrethrum (Tanacetum cinerariifolium (Trevir.) Schultz Bip.) | |
| Translated title | Tkivna kultura bolhača (Tanacetum cinerariifolium (Trevir.) Schultz Bip.) | |
| Type | članek | |
| Source | Acta Biol Slov | |
| Vol. and No. | Letnik 47, št. 2 | |
| Publication year | 2004 | |
| Volume | str. 45-56 | |
| Language | eng | |
| Abstract | Pyrethrum (Tanacetum cinerariifolium), is plant species with the highest amount of natural insecticides - pyrethrins. An in-house information system for the development of different plant tissue cultures and marketing of their products was designed. The application of the relational information system in setting up a research hypothesis of high probability is discussed. By processing the information system, plant tissue cultures' parameters were identified, selected and modified. They were tested on the plant tissue culture of Tanacetum cinerariifolium. The influence of jasmonic acid on axillary shoot differentiation was studied. An inhibitory effect of jasmonic acid on shoot tissue culture differentiation was proven (100 microM, 10 microM), and a prediction method for determination of the variable optimal concentration interval was presented (between 0,5 and 4,5 microM). In addition a HPLC method was introduced for pyrethrins determination. | |
| Summary | Bolhač Tanacetum cinerariifolium je rastlinska vrsta z največjo vsebnostjo naravnih insekticidov - piretrinov. Postavili smo informacijski sistem za razvoj različnih sistemov tkivnih kultur in trženje njihovih proizvodov. Preučevali smo uporabo relacijskega informacijskega sistema za vpeljavo znanstvene hipoteze z visoko verjetnostjo. Z njegovo uporabo smo prepoznali, izbrali in priredili parametre sistema tkivnih kultur. Kot poskusni sistem smo uporabili tkivno kulturo bolhača in ugotavljali vpliv jasmonske kisline (JA) na tvorbo zalistnih poganjkov v tkivni kulturi. Dokazan je bil zaviralni učinek JA na diferencijo kulture poganjkov (100 microM, 10 microM) in predstavljena metoda za napoved intervala spreminjanja optimalne koncentracije JA (med 0,5 in 4,5 `M). Uvedli smo metodo HPLC za ugotavljanje vsebnosti piretrinov. | |
| Descriptors | PYRETHRUM PYRETHRINS PLANT GROWTH REGULATORS TISSUE CULTURE |