Author/Editor		Čebašek, V; Kubinova, L; Ribarič, S; Eržen, I
Title		A novel staining method for quantification and 3D visualisation of capillaries and muscle fibres
Type		članek
Source		Eur J Histochem
Vol. and No.		Letnik 48, št. 2
Publication year		2004
Volume		str. 151-7
Language		eng
Abstract		The aim of this study was to introduce a combined fluorescent staining that clearly demonstrates capillaries and distinguishes them from the basal lamina of muscle fibres in skeletal muscle tissue. The triple staining with CD31, Griffonia (Bandeira) simplicifolia lectin (GSL I) and laminin I efficiently distinguishes vascular endothelium from the basal lamina of skeletal muscle fibres in physiological and pathological conditions. The presented triple staining method has several advantages, which facilitate quantitative analysis of the capillary network, and its relation to individual muscle fibres.
Descriptors		MUSCLE, SKELETAL CAPILLARIES FLUORESCENT DYES MUSCLE FIBERS RATS, WISTAR DYES