Author/Editor		Drobne, Damjana; Milani, Marziale; Zrimec, Alexis; Berden-Zrimec, Maja; Tatti, Francesco; Drašlar, Kazimir
Title		Focused ion beam/scanning electron microscopy studies of Porcellio scaber (Isopoda, Crustacea) digestive gland epithelium cells
Type		članek
Source		Scanning
Vol. and No.		Letnik 27
Publication year		2005
Volume		str. 30-4
Language		eng
Abstract		The focused ion beam (FTB) was used to prepare cross sections of precisely selected regions of the digestive gland epithelium of a terrestrial isopod P scaber (Isopoda, Crustacea) for scanning electron microscopy (SEM). The FIB / SEM system allows ad libitum selection of a region for gross morphologic to ultrastructural investigation, as the repetition of FIB/SEM operations is unrestricted. The milling parameters used in our work proved to be satisfactory to produce serial two-dimensiunal (2-D) cuts and/or three-dimensional (3-D) shapes on a submicrumeter scale. A final, cleaning mill at lower ion currents was employed to minimize the milling artifacts. After cleaning, the milled surface was free of filament- and ridge-like milling artifacts. No other effects of the cleaning mill were observed.
Descriptors		DIGESTIVE SYSTEM CRUSTACEA MICROSCOPY, ELECTRON, SCANNING