| Author/Editor | Štempelj, Mateja | |
| Title | Mehanizem sproščanja histamina iz mastocitov podgane po aktivaciji z živčnim rastnim dejavnikom | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 2004 | |
| Volume | str. 78 | |
| Language | slo | |
| Abstract | Mast cells play a central role in the inflammatory and allergic reactions. In response to immunologic or non-immunologic stimuli they release histamin and a variety of inflammatory and immuno-regulatory mediators. Nerve growth factor (NGF) is essential for the survival of sensory and sympathetic neurones and has a wide range of other effects. It has been shown, that NGF induces degranulation of mast cells by interacting with its high-affinity receptor of a TrkA-type. In the first group of our experiments, we studied the effect of extracellular Na+ and Ca2+ ions on NGF induced histamine release from mast cells. We have found that the removal of extracellular Na+ ions reduces histamine release induced by NGF in the medium containing physiological (1 mM) concentration of Ca2+ ions. In contrast, in the medium containig low (0.1 mM) concentration of Ca2+ ions, the removal of Na+ ions from the medium enhances histamine release elicited by NGF. In the second group of experiments, we evaluated the effect of amiloride, an inhibitor of Na+/Ca2+ exchanger, and 5-N,N-hexamethylene amiloride, an inhibitor of Na+/H+ exchanger, on NGF induced histamine release. Our results indicate, that changing the concentration of extracellular Na+ and Ca2+ ions regulates the activity of Na+/Ca2+ and Na+/H+ exchangers in mast cell membrane. Na+/Ca2+ exchanger regulates the concentration of cytosolic calcium, whereas Na+/H+ exchanger plays an important role in intracellular pH regulation. Combined action of these exchange mechanisms serves to modify histamine release from mast cells. (Abstract truncated at 2000 characters) | |
| Descriptors | MAST CELLS HISTAMINE RELEASE NERVE GROWTH FACTORS HISTAMINE CALCIUM SODIUM AMILORIDE PROTEIN KINASE C PROTEIN-TYROSINE KINASE PHOSPHOLIPASE C CALMODULIN-DEPENDENT PROTEIN KINASES RATS, WISTAR |