| Author/Editor | Kužner, Jernej; Drevenšek, Gorazd; Geršak, Borut; Budihna, Metka | |
| Title | Hypoxic and pharmacological preconditioning preserves vasomotor response of porcine coronary artery | |
| Type | članek | |
| Source | Pol J Pharmacol | |
| Vol. and No. | Letnik 56 | |
| Publication year | 2004 | |
| Volume | str. 789-97 | |
| Language | eng | |
| Abstract | Vasomotor response of the coronary artery depends on both endothelial and smooth muscle cells. Response is altered by hypoxia-reoxygenation induced damages. Hypoxic preconditioning and pharmacological preconditioning as well can prevent these alterations. We compared the effectiveness of both types of preconditioning against hypoxia-reoxygenation-induced changes in vasomotor response of the isolated artery. Porcine arterial rings (3-4 mm wide) were cut from the left auterior descending porcine coronary artery and placed in Krebs-Henseleit solution. In order to obtain control response of the arteries, we contracted arterial rings with 20 mM KCI before ("standard contraction") and after 60-min hypoxia and 30-min reoxygenation. In other groups, nitric oxide-synthase and cyclooxygenase were inhibited. Then, the rings were pre-contracted with U46619 and relaxed by cumulative addition of the substance P. Contractions and relaxations of nonpreconditioned and hypoxically or pharmacologically preconditioned rings were compared. Hypoxic preconditioning was performed by two periods of 5-min hypoxia and 10-min reoxygenation. For pharmacological preconditioning, we used application of adenosine, adrenaline, acetylcholine and angiotensin ll. Analysis was performed with one-way ANOVA, followed by Dunnett's Multiple Comparison Test. After hypoxia-reoxygenation, in nonpreconditioned rings KCI-induced contractions were significantly increased compared to standard contraction. Relaxations of hypoxically and pharmacologically preconditioned rings (expressed as percentages of U46619-induced pre-contraction) were significantly decreased (p < 0.01) compared to hypoxic but not to normoxic rings. Hypoxic and pharmacological preconditioning may preserve contraction and endothelium-dependent relaxation of porcine coronary artery after long-lasting hypoxia-reoxygenation. | |
| Descriptors | CORONARY VESSELS ANOXIA VASOCONSTRICTION POTASSIUM CHLORIDE SUBSTANCE P ADENOSINE EPINEPHRINE ACETYLCHOLINE ANGIOTENSIN II VASODILATION ANALYSIS OF VARIANCE SWINE |