Author/Editor		Petelin, Milan; Naruishi, Koji; Shiomi, Noboyuki; Mineshiba, Junji; Arai, Hideo; Nishimura, Fusanori; Takashiba, Shogo; Murayama, Yoji
Title		Systemic up-regulation of sTNFR2 and IL-6 in Porphyromonas gingivalis pneumonia in mice
Type		članek
Source		Exp Mol Pathol
Vol. and No.		Letnik 76
Publication year		2004
Volume		str. 76-81
Language		eng
Abstract		Aspiration pneumonia is a common cause of death in older people, and the pathophysialogy is a chronic respiratory failure with a mild airway inflammation. In this study, we established a mild inflammatory pneumonia model using Porphyromonas gingivalis (Pg) pathogen-infected mice.It elucidated the effects of Pg-infected pneumonia on proinflammatory cytokines tumor necrosis factor (TNF)-alpha, interleukin-6 (IL-6), and IL-1beta production in both lung tissue and serum. We also elucidated production of soluble (s) TNF receptor (R)s, because TNF-alpha is considered to be a dominant inflammatory mediator. Lung TNF-alpha Ievels significantly increased at 2 h after infection, and rapidly returned to basal level at 24 h. Consistent with increase of TNF-alpha, remarkable increase of sTNFR2 but not sTNFR1 was detected in lung tissue from 2 to 72 h. Interestingly, sTNFR2/sTNFR1 ratio was significantly enhanced at 2 h in serum. In addition, lung IL-1beta and IL-6 levels also significantly increased from 2 to 24 h. Importantly, we found that IL-6 Ievels in serum reflected its local Ievel. These results may suggest that systemically produced sTNFR2 and IL-6 could be a key role to modulate proinflammatory activities of TNF-alpha in Pg-induced lung inflammation simulated aspiration pneumonia.
Descriptors		BACTEROIDACEAE INFECTIONS ANTIGENS, CD PORPHYROMONAS GINGIVALIS INTERLEUKIN-6 PNEUMONIA, ASPIRATION DISEASE MODELS, ANIMAL ENZYME-LINKED IMMUNOSORBENT ASSAY INTERLEUKIN-1 RECEPTORS, TUMOR NECROSIS FACTOR MICE, NUDE