Author/Editor		Koković, Ira
Title		Napovedna vrednost himeričnih transkriptov SYT-SSX iz parafinskih vzorcev sinovijskega sarkoma
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2005
Volume		str. 104
Language		slo
Abstract		Synovial sarcoma is a highly malignant soft tissue tumor, which predominantly occurs in young adults and children. Histologically, biphasic synovial sarcomas are clearly identifiable due to their characteristic biphasic morphology. However, monophasic and poorly differentiated tumors are often difficult to diagnose solely on histological and clinical grounds. With the discovery of specific chromosomal translocation t(X;18)(pll;qll) and chimeric SYT-SSX genes in synovial sarcoma new possibilities for more specific diagnosis and for additional studies of this tumor appeared. Fusion transcript SYT-SSX is an extremely sensitive diagnostic marker found in epithelial as well as in spindle cells of synovial sarcoma regardless of the histologic type. The aim of our study was: a) to introduce the RT-PCR assay for detection of SYT-SSX fusion transcripts from formalin-fixed, paraffin-embedded synovial sarcoma; b) to evaluate diagnostic and prognostic significance of SYT-SSX fusion transcripts; c) to search for possible new characteristics in our study group. Thirty seven patients with synovial sarcoma diagnosed and treated at Institute of Oncology, Ljubljana, Slovenia, and 14 patients from UMC St Radboud, Nijmegen, the Netherlands, have been included in the study. We introduced the RT-PCR assay for detection of SYT-SSX fusion transcripts from fresh-frozen (-70°C) and formalin-fixed, paraffin-embedded synovial sarcoma. The SYT-SSX fusion transcripts were detected in 47/50 (94%) of tumors with adequate RNA for RT-PCR analysis. Contrary to our expectations and some other studies, we could not confirm strong association of the fusion type and histology observed previously. Furthermore, we could not confirm the impact of the fusion type or any clinico-pathological variable on survival of patients with synovial sarcoma. (Abstract truncated at 2000 characters).
Descriptors		SARCOMA, SYNOVIAL TRANSLOCATION (GENETICS) CHIMERA PROGNOSIS POLYMERASE CHAIN REACTION