Author/Editor     Lepoša, Sonja; Vrbnjak, Marko
Title     Vpliv mejozo-aktivirajočega sterola folikularne tekočine (FF-MAS) na kakovost jajčne celice v postopku zunajtelesne oploditve
Type     monografija
Place     Ljubljana
Publisher     Univerza v Ljubljani, Medicinska fakulteta
Publication year     2004
Volume     str. 46
Language     slo
Abstract     Background: FF-MAS or follicular fluid meiosis-activating 4,4 dimethylsterol is an intermediate of cholesterol biosynthesis. Its ability to reinitiate meiosis, arrested by physiological agent hypoxanthine, has by now been well established. Recent studies have shown a potent ability to promote nuclear and cytoplasmatic maturation by culturing animal oocytes in a solution, containing physiological levels of FF-MAS. Although, its role considering oocyte maturation in humans, has not yet been surveyed. Objective: Our aim was to evaluate the impact of FF-MAS and progesterone in the follicular preovulatory period and quality of oocytes in gonadotrophine stimulated cycles in women undergoing IVF. There is no data considering FF-MAS studies, surveying humans ex vivo. Hypothesis: 1. An administration of hCG in women is followed by a time-dependant follicular fluid FF-MAS concentration rise. 2. An administration of hCG causes a follicular fluid cholesterol level to drop. 3. An administration of hCG is followed by an oocyte quality-dependant follicular fluid FF-MAS rise. 4. Administration of hCG is followed by an oocyte quality-dependant follicular fluid (FF) progesterone rise. Materials and methods: 14 females undergoing in vitro fertilisation (IVF) participated in the study. In 11 subjects the preterm follicular aspiration of one ovary has been performed 10-14 h after HCG administration due to preventing an ovarian hyperstimulation syndrome. Later, 34-38 h after hCG administration, oocytes and follicular fluids were retrieved from all 14 subjects. Oocytes were checked for quality and ranked as degenerated, immature and normal metaphase II stage oocytes. In preterm aspiration, all aspirated follicular fluids (FF) were collected and stored together. In term aspiration, FF from each follicle was aspirated and stored separately. All 254 FF aspirates were analysed by HPLC for FF-MAS, progesterone and cholesterol. (Abstract truncated at 2000 characters).
Descriptors     FERTILIZATION IN VITRO
OOCYTES
FOLLICULAR FLUID
MEIOSIS
MATURATION-PROMOTING FACTOR
PROGESTERONE
CHOLESTEROL
OOGENESIS
STEROLS
CHROMATOGRAPHY, HIGH PRESSURE LIQUID