Author/Editor		Ziherl, Kristina; Živec, Katarina
Title		Študij fluidnosti eritrocitnih membran z elektronsko paramagnetno resonanco pri onkoloških bolnikih
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2004
Volume		str. 55
Language		slo
Abstract		Bockground. Many researches indicate that higher fluidity of plasma membranes is a charasteristic of most cancerous cells. This should be a result of lower concetration of cholesterol in cancer cells' membranes. Because of fast division, cancer cells use more cholesterol for building their cell membranes. That's why hipocholesterolemia in cancerous patients is expected and frequent phenomenon. It is however not clear how this influences the fluidity of erythrocyte membranes in cancerous patients. We know that red blood cells lack organells and inner membranes and with that any possibility of endogenous syntesis of lipids. Changes in serum cholesterol concentrations could be shown in lipid composition of red blood cell membrane, which could affect fluidity of these membranes. Aim. To value fluidity of erythrocyte membranes in patients with lung and higher digestive tract cancer and persons without a disease in relation to age, type of cancer and stage. Change in erythrocyte membrane fluidity could be used as a tumor marker. Hypothesis. Erythrocyte membranes of patients with lung and higher digestive tract cancer have higher fluidity. Methods. In prospective study we inclueded 49 patients with lung and higher digestive tract cancer and 25 healthy controls. We have isolated erythrocytes from the blood and studied membrane fluidity with electronic paramegnetic resonance (EPR). To do so, we incorporated spin label in red blood cells' membranes, which gives us an information about fluidity in the neighbourhood. From EPR spectra, we evaluated a few parameters that are in relation with fluidity. These are : empirical correlation time (tauc), which we evaluated at 37°C and order parameter (S). Higher values of these parameters mean less fluid membranes and in relation higher microviscosity. Besides, we also evaluated ratio between parts of spectrum that belong to less and more ordered domains of membrane (H13). (Abstract trunacted at 2000 characters).
Descriptors		ERYTHROCYTE MEMBRANE CELL MEMBRANE PERMEABILITY LUNG NEOPLASMS ESOPHAGEAL NEOPLASMS STOMACH NEOPLASMS ELECTRON SPIN RESONANCE SPECTROSCOPY CHOLESTEROL LIPOPROTEINS, HDL CHOLESTEROL LIPOPROTEINS, LDL CHOLESTEROL TRIGLYCERIDES