| Author/Editor | Čebašek, Vita | |
| Title | Prilagoditev kapilarne mreže skeletnih mišic podgane po poškodbi živca | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 2004 | |
| Volume | str. 88 | |
| Language | slo | |
| Abstract | A detailed knowledge of capillary network density and its geometrical properties is important for understanding the relation between its structure and function, especially if changes in the microcirculation are studied. The aim of our study was to study changes in the capillary network density of skeletal muscle two weeks after nerve-cut and four weeks after nerve-crush. We developed a new staining technique and applied new stereological methods for the evaluation of capillary network density in thick tissue slices. We introduced a new, triple-staining method for simultaneous visualization of muscle fibres and capillaries and evaluated the length of capillaries, the surface area and volume of the muscle fibers in thick tissue slices with novel stereological methods. The capillary endothelium was double-stained; using the antibodies against CD 31 and lectine Griffonia Simplicifolia I (GSL I) and visualized in green fluorescence. Tha basal lamina of the capillaries and muscle fibrils was stained, using antibodies against laminin and visualized in red fluorescence. This staining is suitable for confocal microscopy. Registered series of optical sections are clear enough for the three-dimensional (3D) tissue visualization and for Quantitative evaluation of each muscle fibre capillary supply. Projections of digital triplet of orthogonal sets of stereological test planes into the series of registered optical sections, successfully replace the tedious cutting of tissue, which previous methods for stereological measurements otherwise require. The new staining technique and the two new stereological software programs, FAKIR for measuring the surface area and volume of fibres, and SLICER, for measuring the capillary length were used to evaluate the capillary density in two different rat muscles, slow soleus muscle (SOL) and fast muscle extensor digitorum longus (EDL). (Abstract truncated at 2000 characters). | |
| Descriptors | MUSCLE, SKELETAL MUSCLE DENERVATION NERVE CRUSH MUSCLE FIBERS CAPILLARIES RATS, WISTAR ENDOTHELIUM, VASCULAR MICROSCOPY, CONFOCAL ENDOTHELIAL GROWTH FACTORS VON WILLEBRAND FACTOR |