Author/Editor		Čižek-Sajko, Mojca; Kovačič, Borut; Vlaisavljević, Veljko
Title		Improved fertilization rate and a birth after intracytoplasmic sperm injection of totally immotile sperm, preselected by the hypo-osmotic swelling test
Type		članek
Source		Gynaecol Perinatol
Vol. and No.		Letnik 13, št. 4 suppl 3
Publication year		2004
Volume		str. 155-8
Language		eng
Abstract		In vitro fenilization procedures can be applied to aid infertile couples with complete asthenozoospermia, i.e. with absence of even a single motile sperm in ejaculate. However, the probability of conception with intracytoplasmic injection of spenn (ICSI), selected at random from an immotile sperm sample, is quite slight. In couples, in which the male partner's ejaculate contained only immotile sperm, the hypo-osmotic swelling (HOS) test was used to select viable sperm for the ICSI procedure. Material and methods. Complete asthenozoospermia as the major cause of infertility was diagnosed in 4 couples, of which two male partners had a special form of complete immotility, a sperm stump defect. Out of the total of 10 hormone-stimulated ICSI cycles, in 6 cycles the sperm for ICSI were chosen randomly, without a HOS test. In 4 cycles, the washed semen samples were incubated in a hypo-osmotic solution and only HOS-positive sperm (i.e. sperm with swollen tail) were used for ICSI. Results. Of 6 ICSI cycles without selection of viable sperm using the HOS test, total fertilization failure was noted in 3. Only a total of 20.7% (6/29) oocytes were fertilized. In all 4 ICSI cycles with performed HOS test prior to sperm injection, embryos for transfer were obtained. An 81.8% (18/221 fertilization rate was registered and one twin pregnancy which resulted in a birth of a healthy singleton. Conclusion. We can confirm that also in cases of complete asthenozoospennia, even in those, combined with stump defect, good fertilization rate and pregnancies can be achieved after selecting the viable sperm by HOS test prior to the ICSI procedure.
Descriptors		OLIGOSPERMIA FERTILIZATION IN VITRO SPERMATOZOA OOCYTES EMBRYO TRANSFER