| Author/Editor | Hasselmayer, Oliver; Braun, Veit; Nitsche, Claudia; Moos, Michael; Rupnik, Maja; von Eichel-Streiber, Christoph | |
| Title | Clostridium difficile IStron CdISt1: discovery of a variant encoding two complete transposase-like proteins | |
| Type | članek | |
| Source | J Bacteriol | |
| Vol. and No. | Letnik 186, št. 8 | |
| Publication year | 2004 | |
| Volume | str. 2508-10 | |
| Language | eng | |
| Abstract | Screening a Clostridium dijficile strain collection for the chimeric element Cd1Stl, we identified two additional variants, dcsignated CdIStl-0 aad CdlStl-III. In in vitro assays, we could prove the self-splicing ribozyme activity of these variants. Structural comparison of all known CdlStl variants led us to define four types of IStrons that wc designated CdIStI-0 through CdlStl-III. Since CdIStl-0 encodes two complete transposase-like proteins (TIpA and TIpB), we suggest that it represents the original genetic element, hypothesized before to have originated by fusion of a group I intron and an insertion sequence element. | |
| Descriptors | CLOSTRIDIUM DIFFICILE GENES, BACTERIAL INTRONS MOLECULAR SEQUENCE DATA MUTAGENESIS, INSERTIONAL POLYMERASE CHAIN REACTION |