Author/Editor		Rajčević, Uroš
Title		Differential analysis of transcriptome and proteome in gastric adenocarcinoma
Translated title		Diferencialna analiza transkriptoma in proteoma pri adenokarcinomu želodca
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Mediciska fakulteta
Publication year		2005
Volume		str. 158
Language		eng, slo
Abstract		Most frequent histological type of gastric cancer, one of the most common malignancies in Slovenia and worldwide, is adenocarcinoma. Stomach adenocarcinoma, used as a model malignancy in our study is, as all neoplastic diseases, a complex multigene and multifactorial disease, where a number of different genetic and environmental factors contribute to a network of changes necessary for tumor initiation, promotion and progression. However, these changes will only be selected for if they manifest survival advantages within protein metabolic and signaling pathways and networks. The genes involved in these alterations include oncogenes, transcription factors, tumor suppressor genes, and others, but not all genetic changes are expressed. Technologies such as DNA microarrays allow large-scale analysis of gene expression, but not all changes in gene expression are reflected at the level of protein expression or function. Because proteins are the molecules that are the functional effectors of cellular processes, analysis of the proteome offers a snapshot of the state of in vivo molecular pathways. Furthermore, analysis of the proteome allows the detection of functionally relevant post-translational modifications, such as phosphorylation, not present at the genomic level. Populations of about 40.000 pure non-malignant and pure tumor cells were collected using laser capture microdissection (LCM) technology from five patients with well-differentiated intestinal type and five patients with poorly-differentiated diffuse tumors. Total RNA and total proteins were extracted from selected populations of the cells. Total RNA was amplified with in-vitro transcription procedures and subjected to microarray analysis. Differential expression of about 20.000 target genes was profiled using HG-U133A microarrays. (Abstract truncated at 2000 characters).
Descriptors		STOMACH NEOPLASMS ADENOCARCINOMA SEQUENCE ANALYSIS, RNA PEPTIDE MAPPING OLIGONUCLEOTIDE PROBES PROTEINS ELECTROPHORESIS, GEL, TWO-DIMENSIONAL POLYMERASE CHAIN REACTION SPECTRUM ANALYSIS, MASS GENE EXPRESSION REGULATION, NEOPLASTIC