Author/Editor     Krašna, Metka
Title     Razvoj in priprava gojenja človeških avtolognih keratinocitov v ex-vivo za kritje tkivnih vrzeli pri opeklinah
Translated title     Development and preparation of ex-vivo cultivation of human autologous keratinocytes in treatmen of burns
Type     monografija
Place     Ljubljana
Publisher     Univerza v Ljubljani, Medicinska fakulteta
Publication year     2005
Volume     str. 103
Language     slo
Abstract     Survival of patients with major burns depends mainly on early grafting of suitable skin coverage. Because these patients usually lack a healthy skin for autografting the only option for healing their burns is a grafting of cultured autologous keratinocytes. The purpose of our study was to produce and characterise an autologous cultured skin substitute. We developed a cultured skin substitute in a defined serum-free medium and biologically evaluated it in vitro. The skin substitute was prepared from autologous keratinocytes and dermal fibroblasts that were grown on a fibrin matrix, which was prepared from a fibrin glue Tissucol®. A whole process was developed with a minimal usage of reagents that contained animal proteins and optimised for a clinical application. The skin substitute was evaluated by measuring a cell growth kinetic with MTT assay and by estimating a doubling time of keratinocytes and fibroblasts. Immunofluorescent and immunohistochemical techniques were used to determine types of skin cells and extracellular proteins. A normal human skin was used as a positive control. (Abstract truncated at 2000 characters).
Descriptors     KERATINOCYTES
FIBROBLASTS
BURNS
SKIN, ARTIFICIAL
CELLS, CULTURED
CULTURE MEDIA
EPIDERMIS
APROTININ
FIBRIN
CELL COUNT
CELL SURVIVAL
MICROSCOPY, CONFOCAL
MICROSCOPY, FLUORESCENCE