Author/Editor | Krašna, Metka | |
Title | Razvoj in priprava gojenja človeških avtolognih keratinocitov v ex-vivo za kritje tkivnih vrzeli pri opeklinah | |
Translated title | Development and preparation of ex-vivo cultivation of human autologous keratinocytes in treatmen of burns | |
Type | monografija | |
Place | Ljubljana | |
Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
Publication year | 2005 | |
Volume | str. 103 | |
Language | slo | |
Abstract | Survival of patients with major burns depends mainly on early grafting of suitable skin coverage. Because these patients usually lack a healthy skin for autografting the only option for healing their burns is a grafting of cultured autologous keratinocytes. The purpose of our study was to produce and characterise an autologous cultured skin substitute. We developed a cultured skin substitute in a defined serum-free medium and biologically evaluated it in vitro. The skin substitute was prepared from autologous keratinocytes and dermal fibroblasts that were grown on a fibrin matrix, which was prepared from a fibrin glue Tissucol®. A whole process was developed with a minimal usage of reagents that contained animal proteins and optimised for a clinical application. The skin substitute was evaluated by measuring a cell growth kinetic with MTT assay and by estimating a doubling time of keratinocytes and fibroblasts. Immunofluorescent and immunohistochemical techniques were used to determine types of skin cells and extracellular proteins. A normal human skin was used as a positive control. (Abstract truncated at 2000 characters). | |
Descriptors | KERATINOCYTES FIBROBLASTS BURNS SKIN, ARTIFICIAL CELLS, CULTURED CULTURE MEDIA EPIDERMIS APROTININ FIBRIN CELL COUNT CELL SURVIVAL MICROSCOPY, CONFOCAL MICROSCOPY, FLUORESCENCE |