Author/Editor		Homan, Matjaž
Title		Prevalencija i značaj određivanja bakterijskih gena cagA i iceA u dječjoj populaciji iz Slovenije zaraženoj s Helicobacter pylori
Type		monografija
Place		Ljubljana
Publisher		Sveučilište u Zagrebu, Medicinski fakultet
Publication year		2004
Volume		str. 52
Language		cro
Abstract		Several Helicobacter pylori genes have been identified that probably play a role in the pathogenesis of infection, such as cagA, vacA and iceA. Data concerning the genetic divergence of H. pylori in children are scarce, mostly because of the small number of children enrolled in the genotyping studies. In our study we determined the prevalence and genetic diversity of cagA and iceA genes in Slovenian pediatric population. In addition the relationship between cagA and iceA genotypes and severity of gastroduodenal diseases in pediatric population was studied. To the best of our knowledge our study included the highest number of pediatric patients studied for the presence of cagA and iceA genes to date. DNA was isolated from 105 gastric biopsies previously used in rapid urease test. Biopsies were obtained from the same number of children. H. pylori cagA and IceA status of the isolates were determined by PCR using type specific primers. Biopsies were patohistologically evaluated according to Updated Sydney Classification Among 105 children infected with H. pylori cagA gen was detected in 68 (64,7%) cases. Allelic genotype iceA1 was identified in 62 (59,0%) and genotype iceA2 in 33 (31,4%) patients. Five biopsy specimens (4,7%) contained both iceA allelic genotypes and in five cases we were not able to determine neither iceA alleles. Significant correlation between cagA and iceA1 status (p=0.04 and p=0.004, respectively) and the severity of antral inflammation scored according to Updated Sydney classification were obtained. In conclusion, in the study, which, according to published data included highest number of pediatric patients studied for cagA and iceA gene to date we clearly demonstrate that infection with cagA and iceA1 H. pylori positive strains in children is associated with higher severity of antral inflammation.
Descriptors		HELICOBACTER INFECTIONS HELICOBACTER PYLORI GENES, BACTERIAL GASTRIC MUCOSA UREASE CHILD BIOPSY GASTROSCOPY POLYMERASE CHAIN REACTION ELECTROPHORESIS, AGAR GEL PROSPECTIVE STUDIES