Author/Editor		Mencinger, M; Panagopoulos, I; Andreasson, P; Lassen, C; Mitelman, F; Aman, P
Title		Characterization and chromosomal mapping of the human TFG gene involved in thyroid carcinoma
Type		članek
Source		Genomics
Vol. and No.		Letnik 41, št. 3
Publication year		1997
Volume		str. 327-31
Language		eng
Abstract		Homology searches in the Expressed Sequence Tag Database were performed using SPYGQ-rich regions as query sequences to find genes encoding protein regions similar to the N-terminal parts of the sarcoma-associated EWS and FUS proteins. Clone 22911 (T74973), encoding a SPYGQ-rich region in its 5' end, and several other clones that overlapped 22911 were selected. The combined data made it possible to assemble a full-length cDNA sequence. This cDNA sequence is 1677 bp, containing an initiation codon ATG, an open reading frame of 400 amino acids, a poly(A) signal, and a poly(A) tail. We found 100% identity between the 5' part of the consensus sequence and the 598-bp-long sequence named TFG. The TFG sequence is fused to the 3' end of NTRK1, generating the TRK-T3 fusion transcript found in papillary thyroid carcinoma. The cDNA therefore represents the full-length transcript of the TFG gene. TFG was localized to 3q11-q12 by fluorescence in situ hybridization. The 3' and the 5' ends of the TFG cDNA probe hybridized to a 2.2-kb band on Northern blot filters in all tissues examined.
Descriptors		THYROID NEOPLASMS POLYMORPHISM (GENETICS) DNA, COMPLEMENTARY BASE SEQUENCE AMINO ACID SEQUENCE IN SITU HYBRIDIZATION, FLUORESCENCE BLOTTING, NORTHERN CLONING, MOLECULAR GENE EXPRESSION PROTEINS