Author/Editor		Ficko, Tanja
Title		Opredelitev razvojnih stopenj megakariopeze s količinskim določanjem molekul mRNA za trombocitno specifične glikoproteine
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2005
Volume		str. 107
Language		slo
Abstract		Platelet glycoproteins play critical role in the formation of platelet plug. The glycoproteins GPIa/IIa, GPIc/IIa and GPIc'/IIa are necessary for platelets to adhere to the site of injury and GPIIb/IIIa plays the main role in aggregation of activated platelets to other platelets via fibrinogen and other ligands. On those glycoproteins, several biallelic systems can be found. Human platelet alloantigens HPA are prone to alloimmune recognition. After transfusion or during the pregnancy alloimmunisation to the HPA antigens can result in post-transfusion refractoriness to platelet transfusions and fetomaternal alloimune thrombocytopenia. Platelet glycoproteins are also specific cell surface markers and can be used for identifying different stages during megakaryopoiesis. The most immature megakaryocytic progenitors BFU-MK express CD34 antigen, the CFU-MK express low amount of GPIIb besides CD34 and three stages of promegakarioblasts differs in the amount of CD34, GPIIb and GPIIIa. Essential thrombocythemia is chronic myeloproliferative disorders whose behavior is govern principally by the abnormal proliferation of a malignant megakaryocytic clone and is characterized by elevated number of megakaryocytes in bone marrow and sustained thrombocytosis. The diagnosis depends largely on the exclusion of myelodysplasia and other chronic myeloproliferative disorders with a thrombocytosis and the absence of the cause for a reactive thrombocytosis. (Abstract truncated at 2000 characters)
Descriptors		THROMBOCYTHEMIA, HEMORRHAGIC MEGAKARYOCYTES BLOOD PLATELETS HEMATOPOIETIC STEM CELLS PLATELET MEMBRANE GLYCOPROTEINS FLOW CYTOMETRY POLYMERASE CHAIN REACTION ANTIGENS, CD36 RNA, MESSENGER GENOTYPE