Author/Editor		Vranac, Tanja
Title		Protitelesni odziv miši BALB/c in Prnp0/0 na izbrane peptidne dele prionskega proteina
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2005
Volume		str. 117
Language		slo
Abstract		It is believed that the main cause for transmissible spongiform encephalopathies (TSE) is the conformational change from the normal cellular prion protein (PrPc) isoform into the abnormal isoform, named prion (PrPssc). While it is known that the PrPsc has a higher B-sheet content, its exact structure has not been determined yet. The two isoforms have the same primary structure, and with PrP being a highly conserved protein among different species, the immune response to PrP is very weak. This immune tolerance represents an obstacle in the development of sensitive diagnostic reagents based on monoclonal antibodies (mAbs) and makes an active anti-TSE vaceine difficult to develop. In theory, the lack of response of the immune system could be overcome by immunisation with short peptide parts of PrP bound to a carrier molecule. In this case, short peptides are able to undertake a variety of conformations. We hypothesised that it should be possible to obtain antibodies specific for conformations that are not present in the native protein. Based on published articles, we inferred which parts of PrP might differ in conformation between PrPc and PrPsc. We expected to obtain different mAbs that would bind to different isoforms of PrP and that some of them would be PrPsc-specific. (Abstract truncated at 2000 characters)
Descriptors		PRIONS HEMOCYANIN CONJUGATION, GENETIC DNA, RECOMBINANT RECOMBINANT FUSION PROTEINS TRANSFORMATION, GENETIC POLYMERASE CHAIN REACTION PROTEIN CONFORMATION ANTIBODIES, MONOCLONAL BASE SEQUENCE BRAIN MICE, INBRED BALB C