Author/Editor		Čebašek, Vita; Eržen, Ida
Title		A triple staining method for simultaneous visualisation of capillaries and muscle fibres in thick tissue slices
Type		članek
Source		In: Čeh M, Dražič G, Fidler S, editors. MCM 2005. Proceedings of the 7th multinational congress on microscopy; 2005 Jun 26-30; Portorož. Ljubljana: Slovene society for microscopy,
Publication year		2005
Volume		str. 391-2
Language		eng
Abstract		The aim of this study was to introduce a combined fluorescent staining that clearly demonstrates capillaries and skeletal muscle fibres in thick tissue slices. The triple staining with CD31, Griffonia (Bandeira) simplicifolia lectin (GSL I) and laminin efficiently distinguishes vascular endothelium from the basal lamina of skeletal muscle fibres in physiological and pathological conditions. The presented staining method is suitable for confocal microscopy and for quantitative analysis of the capillary network in relation to individual skeletal muscle fibres. Keywords: capillaries, confocal microscopy, immunohistochemistry, skeletal muscle.
Descriptors		CAPILLARIES MUSCLE FIBERS MUSCLE, SKELETAL MICROSCOPY, CONFOCAL RATS, WISTAR