Author/Editor		Arrigler, Vesna; Kogej, Ksenija; Majhenc, Janja; Svetina, Saša
Title		Interaction of cetylpiridinium chloride with giant lipid vesicles
Type		članek
Source		Langmuir
Vol. and No.		Letnik 21
Publication year		2005
Volume		str. 7653-61
Language		eng
Abstract		The interaction of cationic surfactant cetylpyridinium chloride, CPC, with giant lipid vesicles prepared from 1-palmitoyl-2-oleoylphosphatidylcholine, POPC was examined at various concentrations of the lipid component. The lipid concentration was determined by a spectrophotometric method. The potentiometric method based on surfactant-selective electrode was used for the determination of surfactant concentration in the external water solution. From these results, moles of surfactant incorporated in the membrane per mole of lipid (parameter beta) and two kinds of partition coefficients were calculated. Their values were found to be considerably larger than the available literature data. A three stage process of surfactant-induced solubilization of lipid vesicles was observed. First, stable mixed bilayers form, which become saturated with CPC at a value betasat larger than 0.8, which then gradually disintegrate. Just prior to the breakdown of the vesicular structure, formation of ellipsoidal vesicles was observed by optical microscopy. This phenomenon was attributed to the cooperative incorporation of surfactant into the bilayer. Fluorescence measurements have shown that the second stage in the solubilization process of POPC by the C16 chainlength surfactant does not involve mixed micelles. These are formed only in the third stage, which is the complete solubilization of POPC bilayers. The corresponding critical micellization concentration decreases with increasing concentration of the lipid component.
Descriptors		LIPID BILAYERS MEMBRANE FLUIDITY CETYLPYRIDINIUM PHOSPHATIDYLCHOLINES MEMBRANES, ARTIFICIAL SPECTROPHOTOMETRY POTENTIOMETRY