Author/Editor     Božič, B; Čučnik, S; Kveder, T; Rozman, B
Title     Avidity of anti-beta-2-glycoprotein I antibodies
Type     članek
Source     Autoimmunity reviews
Vol. and No.     Letnik 4
Publication year     2005
Volume     str. 303-8
Language     eng
Abstract     The terms affinity and avidity are often used indiscriminately, despite clearly differing. Since affinity refers to monovalent binding of antibodies to a monovalent epitope, the majority of data on the binding of anti-beta2-glycoprotein I antibodies (anti-beta2-GPI) characterized their avidity rather than affinity. Anti-beta2-GPI were generally believed to be of low avidity, but heterogeneous avidity of patients' IgG anti-beta2-GPl has been demonstrated. High avidity anti-beta2-GPI monoclonals were reported to possess higher pathogenicity than low avidity anti-beta2-GPI. Polyclonal high avidity anti-beta2-GPI were found to be more common in patients with antiphospholipid syndrome (APS) and associated with thrombosis. Some conformational changes of beta2-GPI are required for the binding of polyclonal anti-beta2-GPI to the antigen: neither high density of the antigen nor high avidity of the anti-beta2-GPI alone is sufficient for the recognition. Avidity of anti-beta2-GPI should be considered in any attempt of inter-laboratory standardisation and/or evaluation of anti-beta2-GPl enzymelinked immunosorbent assay (ELISA).
Descriptors     ANTIPHOSPHOLIPID SYNDROME
LUPUS ERYTHEMATOSUS, SYSTEMIC
AUTOANTIBODIES
GLYCOPROTEINS
ANTIBODY AFFINITY
ANTIBODIES, MONOCLONAL
IGG
ENZYME-LINKED IMMUNOSORBENT ASSAY