Author/Editor     Čučnik, S; Božič, B; Kveder, T; Tomšič, M; Rozman, B
Title     Avidity of anti-beta2-glycoprotein I and thrombosis or pregnancy loss in patients with antiphospholipid syndrome
Type     članek
Source     Ann NY Acad Sci
Vol. and No.     Letnik 1051
Publication year     2005
Volume     str. 141-7
Language     eng
Abstract     We aimed to evaluate avidity of anti-beta2-glycoprotein I antibodies (anti-beta2-GPIs) in patients with antiphospholipid syndrome (APS) at the time of acute thrombotic events or pregnancy loss as compared with clinical event free periods. To do so, 69 sera samples from 16 patients (6 with primary APS and 10 with APS secondary to systemic lupus erythematosus ) were selected on the basis of anti-beta2-GPI positivity. Avidity of IgG anti-beta2-GPIs was determined by chaotropic enzyme-linked immunosorbent assay (ELISA), using increased NaCI concentration during antibody binding. High, heterogeneous, and low-avidity anti-beta2-GPIs were measured in APS patients, with no clear pattern regarding the time of thrombotic events or pregnancy failure. In general, anti-beta2-GPI avidity did not change substantially during disease course. We concluded that avidity of anti-beta2-GPIs appears to be a rather stable parameter in an individual APS patient. Considering the previously shown association of high-avidity anti-beta2-GPIs with venous thrombosis, avidity of anti-beta2-GPIs may be a better predictor of predisposition to thrombosis and unsuccessful pregnancy than levels of antiphospholipid antibodies, which may fluctuate over time owing to several factors.
Descriptors     ANTIPHOSPHOLIPID SYNDROME
LUPUS ERYTHEMATOSUS, SYSTEMIC
THROMBOSIS
ABORTION
ANTIBODY AFFINITY
IGG
ANTIBODIES, ANTICARDIOLIPIN
GLYCOPROTEINS