| Author/Editor | Tomič, Viktorija | |
| Title | Primerjava metod za dokazovanje bakterij Staphylococcus aureus odpornih proti meticilinu (MRSA) | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 2005 | |
| Volume | str. 84 | |
| Language | slo | |
| Abstract | Staphylococcus aureus is one of the most important pathogens causing infections in hospitals as well as in the community. For successful treatment of staphylococcal infections it is very important to determine resistance to methicillin very early in the course of treatment, since resistance to methicillin in clinical sense means resistance to all beta-lactam antibiotics. Methicillin-resistant S. aureus (MRSA) is well established as an infection control problem in hospitals and long-term care facilities all over the world. For infection control programme in the hospital to be successful, early and reliable laboratory detection of MRSA from screening specimens of patients with risk factors for colonization is of utmost importance. The aim of our study was to find the combination of tests that would provide reliability, shortest time possible for end result and affordability concerning the money spent for each test for the routine detection of MRSA. In microbiology laboratory problems may arise already when trying to identify colonies which resemble staphylococci. To determine resistance to methicillin majority of laboratories still use different phenotipical methods, which are easy to perform, cheap, do not need special, expensive equipment, but they are relatively slow and results may be influenced by different physical and chemical factors (eg. temperature, pH, salt concentration, etc). False identification of bacterial strain and/or false determination of resistance to methicillin can have serious clinical, epidemiological as well as financial concequences. In our study we have compared 7 different tests for identification of S. aureus and 6 different tests for methicillin resistance determination. All results were compared to the results we acquired by PCR (polymerase chain reaction) identification of S. aureus and detection of mecA gen. (Abstract truncated at 2000 characters) | |
| Descriptors | STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE AGGLUTINATION TESTS LATEX FIXATION TESTS COAGULASE DEOXYRIBONUCLEASES POLYMERASE CHAIN REACTION NUCLEIC ACID HYBRIDIZATION MICROBIAL SENSITIVITY TESTS OXACILLIN |