Author/Editor | Štempelj, M; Ferjan, I | |
Title | Signaling pathway in nerve growth factor induced histamine release from rat mast cells | |
Type | članek | |
Source | Inflamm Res | |
Vol. and No. | Letnik 54 | |
Publication year | 2005 | |
Volume | str. 344-9 | |
Language | eng | |
Abstract | Objective and Design: We investigated a signal transduction pathway involved in NGF induced histamine secretion from mast cells. We compared this mechanism with the exocytosis induced by basic secretagogue compound 48/80. Materials and Methods: Isolated rat peritoneal mast cells were obtained from male Wistar rats. Histamine release was assayed spectrofluorometrically. Results: We found that tyrosine kinase inhibitor genistein, phospholipase C (PLC) inhibitor U-73122, phosphatidylinositol 3-kinase (PI-3 kinase) inhibitor wortmannin, protein kinase C (PKC) inhibitors, staurosporine and GF109203X, but not MAP kinase inhibitors, SB203580 and PD98059, reduce histamine secretion in NGF provoked mast cell degranulation. In compound 48/80 mediated degranulation, we confirmed only the involvement of tyrosine kinase and PLC, but not PI-3 kinase, PKC and MAP kinases. Conclusions: Our results indicate that release of histamine from mast cells after stimulation with NGF is regulated by tyrosine kinase, PLC, PI-3 kinase and PKC, but not by MAP kinases. This biochemical pathway differs from that provoked by compound 48/80. | |
Descriptors | SIGNAL TRANSDUCTION HISTAMINE MAST CELLS MALEIMIDES INDOLES RATS, WISTAR PROTEIN KINASE C PYRIDINES PYRROLIDINONES STAUROSPORINE TYROSINE IMIDAZOLES FLAVONES ESTRENES ENZYME INHIBITORS DOSE-RESPONSE RELATIONSHIP, DRUG CELLS, CULTURED ANDROSTADIENES ANALYSIS OF VARIANCE |