Author/Editor		Klemenc, P; Skalerič, U; Artnik, B; Nograšek, P; Marin, J
Title		Prevalence of some herpesviruses in gingival crevicular fluid
Type		članek
Source		J Clin Virol
Vol. and No.		Letnik 34
Publication year		2005
Volume		str. 147-52
Language		eng
Abstract		Background: The herpesviruses, ancient pathogens which have co-evoluted with human, are etiologically associated with a number of discases, from asymptomatic to oncogenic and mortal discases. It seems that some of them have also an important role in the pathogencsis of human periodontal discasc. Objective: This study aimed to determinc the prevalence of Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), human herpesvirus 8 (HHV-8) and human cytomegalovirus (HCMV) in gingival crevicular ftuid (GCF) and, eventually, to find the correlation between specific virus types and clinical parameters which are important in periodontitis, like plaque index (PI), gingival index (GI) and probing depth (PD). Study design: A polymerase chain reaction (PCR) and digestion of PCR products with restriction endonucleasc were employed to identify the presence of EBV, HHV-6, HHV 8 and HCMV. Results: Out of 66 samples of GCF taken from the patients with periodontal disease, EBV was found in 29 (43.9%), HHV-6 in 16 (24.2%) and HCMV in 2 (3%) samples, while in the samples of healthy persons, these viruses were not found. HHV-8 was detected neither in the patients with periodontitis nor in healthy control group. More positive results were found in clinical samples taken from people with higher PI and GI and in the samples taken from the patients with medium PD (PD=3-6 mm). In all HHV-6 positive samples, we found only variant A; as for EBV positive samples, type A and type B were identified and also co-infection with the two types. It seems that there is a correlation between PI, PD and EBV types, but no correlation was found between EBV types and GI or HHV-6 types and PI, PD, GI. Conclusions: "the present findings confirm some association between herpesviruses and human periodontitis.
Descriptors		PERIODONTAL DISEASES GINGIVAL CREVICULAR FLUID HERPESVIRIDAE INFECTIONS HERPESVIRUS 4, HUMAN HERPESVIRUS 6, HUMAN CYTOMEGALOVIRUS DENTAL PLAQUE INDEX PERIODONTAL INDEX POLYMERASE CHAIN REACTION