Author/Editor		Keše, Darja
Title		Uporaba molekularnih metod v diagnostiki okužb z bakterijo Chlamydia trachomatis
Translated title		Application of molecular methods in diagnostics of Chlamydia trachomatic infection
Type		članek
Source		In: Miljković J, editor. Strokovno srečanje z mednarodno udeležbo 3. dermatološki dnevi; 2005 nov 11-12; Maribor. Maribor: Splošna bolnišnica Maribor,
Publication year		2005
Volume		str. 39-45
Language		slo
Abstract		For many years, isolation in tissue culture was considered the test of choice for laboratory diagnosis of Chlamydia trachomatis infection. Introduction of non-culture tests, such as direct immunofluorescence and enzymeimmunoassay for detection of chlamydia antigens in clinical specimens, made chlamydia diagnostic tests more widely available. Recently introduced molecular assays turned out to be more sensitive as culture methods and offer a better approach for the rapid detection of these pathogens. The broad spectrum of established and well evaluated commercial test systems for molecular detection of C trachomatis has yet been created for routine use in clinical microbiology laboratories. The major targets for amplification based tests against C. trachomatis are generally the cryptic chlamydial plasmid or rRNA. We can conclude that molecular diagnostic techniques which use PCR have proven to be an important tool for the rapid etiologic diagnosis of C. trachomatis infections.
Descriptors		CHLAMYDIA INFECTIONS CHLAMYDIA TRACHOMATIS FLUORESCENT ANTIBODY TECHNIQUE, DIRECT RNA, BACTERIAL POLYMERASE CHAIN REACTION ENZYME-LINKED IMMUNOSORBENT ASSAY