| Author/Editor | Hes, O; Vaneček, T; Perez-Montiel, DM; Cabrero-Alvarado, I; Hora, M; Suster, S; Lamovec, J; Čurik, R; Mandys, V; Michal, M | |
| Title | Chromophobe renal cell carcinoma with microcystic and adenomatous arrangement and pigmentation - a diagnostic pitfall. Morphological, immunohistochemical, ultrastructural and molecular genetic report of 20 cases | |
| Type | članek | |
| Source | Virchows Arch | |
| Vol. and No. | Letnik 446 | |
| Publication year | 2005 | |
| Volume | str. 383-93 | |
| Language | eng | |
| Abstract | We present clinical, morphological, immunohitochemical, ultrastructural and molecular genetic features of 20 cases of a peculiar form of chromophobe renal carcinoma (CRCC) with morphology differing from that of conventional CRCC. Microscopically, the typical features of the tumors were microcystic arrangement and formation of adenomatous structures. Microcystic areas were composed of smaller cosinophilic and bigger pale cells having cytological appearance typical of conventional CRCC. Cytological features of the adenomatous structures were mostly different from those of conventional CRCC. They had a typical columnar arrangement with nuclei positioned at the base of the glandular structures and a small amount of a deeply eosinophilic cytoplasm often endowed with brush border facing the lumen of the glands. In addition, all the tumors showed a brown pigmentation. The pigmentation was located mostly extracellularly, where it formed pools of heavy deposits. Microscopic calcifications present in all cases formed psammoma bodies or else the calcifications were more extensive and amorphous in shape. Ultrastructurally. the cells showed features characteristic of CRCC: typical cytoplasmic vesicles were 100-700 nm in size and mitochondria had tubulovesicular, lamellar or circular cristae. Some tumor cells contained dark, variously sized electron-dense pigment granules. Neither melanosomes nor membrane-bound neurosecretory granules were seen. Using fluorescence in-situ hybridization probes for chromosomes 1, 2, 6, 10, 13, 17 and 21, the tumors revealed massive loss of tested chromosomes typical for conventional CRCC. Monosomy of chromosomes 1, 2, 6, 10, 13 and 21 was found in 100, 36, 91, 82, 82, 82 and 64% of cases, respectively. None of the cases showed mutation of axons 9. 11, l3 and 17 of the c-kit gene. (Abstract truncated at 2000 characters) | |
| Descriptors | KIDNEY NEOPLASMS ADENOMA, OXYPHILIC CARCINOMA, RENAL CELL SEX FACTORS AGE FACTORS PIGMENTS IN SITU HYBRIDIZATION, FLUORESCENCE FOLLOW-UP STUDIES DIAGNOSIS, DIFFERENTIAL DNA MUTATIONAL ANALYSIS CYTOPLASM |