Author/Editor		Zidar, Nina; Jeruc, Jera; Balažic, Jože; Štajer, Dušan
Title		Neutrophils in human myocardial infarction with rupture of the free wall
Type		članek
Source		Cardiovasc Pathol
Vol. and No.		Letnik 14, št. 5
Publication year		2005
Volume		str. 247-50
Language		eng
Abstract		Introduction Experimental studies have shown that neutrophils might play an important role in the pathogenesis of ischemic and reperfusion injury in myocardial infarction (MI). Our aim was to compare histologic characteristics of MI with and without rupture of the free wall (RFW), with emphasis on the density of interstitial neutrophil infiltration. Methods Autopsy samples of infarcted heart tissue from 110 patients with MI (50 with and 60 without RFW) were included. On the basis of histologic changes and clinical data, all cases were divided into three groups according to the duration of MI (<- 1 day, 1-7 days, and I-4 weeks). Neutrophils were stained immunohistochemically with antibodies against CD15. The intensity of interstitial neutrophil infiltration was determined on the basis of percentage of the infiltrated myocardial area using an image analysis system. Results In MI that were less than 1 day or more than 7 days old, we did not observe any differences in histologic characteristics between cases with and those without RFW. In MI that were more than 1 day and less than 7 days old, we observed a significantly more intensive interstitial neutrophil infiltration in cases with RFW than in those without RFW. However, there were no significant differences in neutrophil infiltration between patients who received reperfusion treatment and those who did not. Conclusions Our results suggest that intensive interstitial neutrophil infiltration in human MI might increase the risk of the RFW between the 2nd and the 7th days when the density of neutrophil infiltration is believed to reach a peak. We failed to confirm the hypothesis based on experimental studies that reperfusion treatment contributes significantly to the density of neutrophil infiltration.
Descriptors		MYOCARDIAL INFARCTION HEART RUPTURE, POST-INFARCTION NEUTROPHILS SEX FACTORS AGED ANTIGENS, CD15 IMMUNOHISTOCHEMISTRY