| Author/Editor | Gornjak-Pogorelc, Barbara; Jazbec, Janez; Zupanič-Pajnič, Irena; Balažic, Jože | |
| Title | Molekularno-genetska analiza himerizma po alogenski transplanatciji kostnega mozga | |
| Translated title | Bone marrow engraftment chimerism monitoring by molecular-genetic (STR) analysis | |
| Type | članek | |
| Source | In: Luzar B, Poljak M, Glavač D, et al, editors. Molekularna diagnostika v medicini. Zbornik 15. spominsko srečanje akademika Janeza Milčinskega, 36. memorialni sestanek profesorja Janeza Plečnika, 1. srečanje Slovenskega društva za humano genetiko z mednarodno udeležbo; 2005 30 nov - 2 dec; Ljubljana. Ljubljana: Medicinska fakulteta, | |
| Publication year | 2005 | |
| Volume | str. 29-41 | |
| Language | slo | |
| Abstract | In the Republic of Slovenia, there are no detailed statutory regulations on the collection of biological samples intended for the analysis of the basic genetic material of a living person without his/her consent and against a person in cases where there are justified grounds that this person committed a criminal offence and where, by such analysis, data necessary for carrying out a successful process will be obtained. In the middle of the 1980s, the development of molecular biology and DNA analysis enabled a new level of quality for forensic medicine in recognising biological traces. The discovery of DNA's useful value signified huge progress in the quality of forensic work in the field of criminal and civil legal procedures. In forensic and legal practice, previous work has indicated the necessity to adopt appropriate and detailed rules to regulate the method for collecting biological samples intended for DNA analysis. The draft of the rules will be precisely presented in the continuation. Key words: forensic science; biological samples; collection; DNA analysis; rules. Monitoring of hematopoietic cell chimerism following bone marrow transplantation is important for establishing graft rejection and disease relapse. Samples were subjected to multiplex PCR using AmpF/STRr IdentifilerTM kit followed by capillary electrophoresis and fluorescent detection. Multiplex PCR offers an additional element of increased specificity by simultaneously analysing multiple STR markers, which is often required when transplants are from related individuals. Percent chimerism was determined as a ratio of allele peak heights measured in RFU between donor and recipient alleles using selected formulas that was chosen according to informativiness of alleles. Percent chimerism was determined per marker and the results for all informative markers were averaged to generate the final percent chimerism result. (Abstract truncated at 2000 characters) | |
| Descriptors | BONE MARROW TRANSPLANTATION HEMATOPOIETIC STEM CELL TRANSPLANTATION CHIMERA MICROSATELLITE REPEATS POLYMERASE CHAIN REACTION |