Author/Editor     Bergant, M; Ambrožič-Dolinšek, J; Demšar, T; Dreo, T; Ravnikar, M; Žel, J; Camloh, M
Title     Effects of antibiotics on contaminated callus cultures of pyrethrum
Type     članek
Source     Phyton-ann Rei Bot A
Vol. and No.     Letnik 45, št. 2
Publication year     2005
Volume     str. 197-206
Language     eng
Abstract     Pyrethrum [Tanacetum circerariifolium (TREVIR.) SCHULTZ-BIP, Asteraceae] is a source of pyrethrins, natural insecticides that are widely used in agriculture and for domestic purposes. In callus cultures of pyrethrum microbial contaminants were observed routinely, therefore it was necessary to use antibiotics to maintain aseptic cultures. The antibiotics, streptomycin, cephalexin and penicillin-G were used for antibiotic pulse treatment. Their effect on callus growth and microbial elimination was investigated.
Descriptors     PYRETHRUM
PLANT DISEASES
ANTIBIOTICS
CELLS, CULTURED
POLYMERASE CHAIN REACTION