Author/Editor | Bergant, M; Ambrožič-Dolinšek, J; Demšar, T; Dreo, T; Ravnikar, M; Žel, J; Camloh, M | |
Title | Effects of antibiotics on contaminated callus cultures of pyrethrum | |
Type | članek | |
Source | Phyton-ann Rei Bot A | |
Vol. and No. | Letnik 45, št. 2 | |
Publication year | 2005 | |
Volume | str. 197-206 | |
Language | eng | |
Abstract | Pyrethrum [Tanacetum circerariifolium (TREVIR.) SCHULTZ-BIP, Asteraceae] is a source of pyrethrins, natural insecticides that are widely used in agriculture and for domestic purposes. In callus cultures of pyrethrum microbial contaminants were observed routinely, therefore it was necessary to use antibiotics to maintain aseptic cultures. The antibiotics, streptomycin, cephalexin and penicillin-G were used for antibiotic pulse treatment. Their effect on callus growth and microbial elimination was investigated. | |
Descriptors | PYRETHRUM PLANT DISEASES ANTIBIOTICS CELLS, CULTURED POLYMERASE CHAIN REACTION |