Author/Editor     Erdani-Kreft, Mateja; Sterle, Maksimiljan; Jezernik, Kristijan
Title     Distribution of junction- and differentiation-related proteins in urothelial cells at the leading edge of primary explant outgrowths
Type     članek
Source     Histochem Cell Biol
Vol. and No.     Letnik 125
Publication year     2006
Volume     str. 475-85
Language     eng
Abstract     Abstract Leading edge cells, which are located at the forefront of a wound margin, play a significant role in coordinating the wound healing process. In this study, leading edge cells of the urothelial explant outgrowth, resembling leading edge cells during urothelial fullthickness wound healing in vivo, were analyzed for expression and distribution of junction and differentiation-related proteins. Ultrastructural and immunofluorescence studies revealed that urothelial cells at the leading edge expressed ZO-1, claudin-4, occludin, E-cadherin, cytokeratin 7 and cytokeratin 20, while no expression of claudin-8 was noted. ZO-1, claudin-4, occludin and E-cadherin were localized along the cell membranes where neighbouring leading edge cells were in contact. Cytokeratin 7 was detected as filaments and cytokeratin 20 as small dots and sparse filaments. In conclusion, we detected early expression of ZO-1, claudin-4 and occludin at the urothelial leading edge, predicating the later formation of tight junctions as a necessary stage for the differentiation process that subsequently begins. The expression of occludin and cytokeratin 20 in urothelial cells at the leading edge suggests that leading edge cells may develop into fully differentiated superficial cells.
Descriptors     BLADDER
UROTHELIUM
WOUND HEALING
INTERCELLULAR JUNCTIONS
CADHERINS
MICE, INBRED STRAINS
KERATIN
MICROSCOPY, ELECTRON
FLUORESCENT ANTIBODY TECHNIQUE
MICROSCOPY, CONFOCAL
CELL DIFFERENTIATION
CELL DIVISION
CELLS, CULTURED
CULTURE MEDIA
ETHANOLAMINES
ADENINE
HYDROCORTISONE
INSULIN
STREPTOMYCIN
PENICILLINS
CALCIUM