Author/Editor		Avguštin, Bojana
Title		Citokinski profil pri bolnikih s shizofrenijo
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2005
Volume		str. 98
Language		slo
Abstract		AIterations of cytokine levels represent the most consistent finding from studies concerning the changes of the immune system in schizophrenia. Most important immunological studies in schizophrenia show inconsistent results regarding Th, and Thz balance. Objectives: We evaluated the in vitro T helper (Th)1 cytokine and Th2 cytokine secretion in patients with acute exacerbation of schizophrenia and compared with the results from healthy subjects. Cytokine profiles were assessed with the in vitro secretion of interferon (IFN)-y and interleukin (IL)-4, which are the most important cytokines of Th1 and Th2 response. The cytokine profiles between different schizophrenia syndromes were compared. The influence of several confounding factors on the in vitro cytokine secretion were assessed. The intracellular concentration of IFN-y and IL-4 in CD4+ T lymphocytes were evaluated with flow cytometry. Methods: Peripheral blood mononuclear cells (PBMC) were isolated from 100 patients with acute exacerbation of schizophrenia and from 34 healthy volunteers. The PBMC were activated with ionomycin (IONO) in combination with phorbol 12-myristate 13-acetate (PMA). After 40-hour incubation, the culture supernatants were collected and concentrations of Th1 and Th2 related cytokines, IFN-y and IL-4 were measured by ELISA. The intracellular cytokine concentrations were compared between 31 patients with acute exacerbation of schizophrenia and 34 healthy volunteers. After stimulation with phorbol 12-myristate 13-acetate and ionomycin CD4+ T cells were examined for the intracellular concentration of IFN-y and IL-4 using intracellular cytokine staining and flow cytometry. Results: After activation with IONO and PMA PBMC from patients with acute exacerbation of schizophrenia produced significantly more IFN-y and IL-4, with shift to the Th2 immune response compared to healthy volunteers. (Abstract truncated at 2000 characters)
Descriptors		SCHIZOPHRENIA ANTIPSYCHOTIC AGENTS TH1 CELLS TH2 CELLS INTERFERON TYPE II INTERLEUKIN-4 CD4 LYMPHOCYTE COUNT DISEASE PROGRESSION FLOW CYTOMETRY