Author/Editor		Poljak, Mario; Kocjan, Boštjan J; Seme, Katja; Babič, Dunja Z
Title		Real-time PCR for detection of parenterally transmissible viruses
Type		članek
Source		In: Berencsi G, Khan AS, Haloužka J, editors. Emerging biological threat. Proceedings of the NATO advanced research workshop. Vol 370; 2003 Oct 5-8; Budapest. Amsterdam: IOS Press,
Publication year		2005
Volume		str. 77-83
Language		eng
Abstract		Although commercially available nucleic acid amplification assays exist for three most important parenterally transmissible viruses, e.g. human immunodeficiency virus type ( and hepatitis A and C viruses, the relatively high price of these assays obviates their use in many parts of the world. Among recent technical improvements in the field of molecular virology, real-time polymerase chain reaction (PCR) is one of the most exciting and is expected to be an alternative and a cheaper diagnostic tool for the detection of parenterally transmissible viruses. Real-time PCR allows a simultaneous amplification and quantification of specific nucleic acid sequences. This is achieved by a combination of rapid thermal cycling and cycle-by-cycle basis detection of the reaction kinetics by means of fluorimetry.
Descriptors		POLYMERASE CHAIN REACTION VIRUS DISEASES HIV-1 HIV-2 HEPATITIS B VIRUS HEPATITIS C-LIKE VIRUSES HTLV-I HTLV-II