| Author/Editor | Kamin, Andreja | |
| Title | Molekularna diagnostika okužb z virusi TT in ugotavljanje prekuženosti pri zdravi slovenski populaciji in hemofilikih | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Univerza v Ljubljani, Medicinska fakulteta | |
| Publication year | 2005 | |
| Volume | str. 66 | |
| Language | slo | |
| Abstract | In 1997 Japanese researchers isolated a viral clone named N22 from a serum of a patient with posttransfusion non-A to E hepatitis. The N22 clone was found to originate in the genome of a DNA virus, which was designated TT virus (TTV) after the initials of the initial patient. TTV genome is a circular, single-stranded DNA. In prototype isolate TA278 it is composed of 3852 nucleotides. TTV is an unenveloped virus with 30-50 nm in diameter. It has a density of 1,26 g/ml as judged from sucrose density gradient centrifugation. Further experiments resulted in isolations of many new TTV variants, which show a great heterogeneity. In general these variants could be clustered into at least four genetic groups. On the basis of genomic organization similarity, TTV was classified into Circoviridae virus family. Clinical importance of TTV has been a matter of extensive investigations for several years now, however it still remains a puzzle. TTV shows a parenteral and enterical route of transmission and its prevalence in all studied populations is relatively high. In prevalence studies, PCR primers of different specificities for TTV DNA amplification are being used, which leads to rather different TTV infection frequency data, and very poor comparability of the existent data. To find out the frequency of TTV infection in Slovenia, we designed a new systematical PCR method for detection of all known TT virus strains. Despite of many publications, no method was described that would enable differentiation between different TTV strains infections from various TTV genetic groups. Our real time PCR method enables us to distinguish between different TTV strains infections from three genetic groups on the basis of different antisense primers, deduced from different TTV genomes. This method is economic due to use of only one Taq-Man probe. It is useful for screening blood plasma, serum or PMBC fraction samples in different populations. (Abstract trunacted at 2000 characters) | |
| Descriptors | HEMOPHILIA BLOOD TRANSFUSION CIRCOVIRIDAE CIRCOVIRIDAE INFECTIONS GENOME, VIRAL POLYMERASE CHAIN REACTION BASE SEQUENCE ELECTROPHORESIS, AGAR GEL SLOVENIA |