Author/Editor | Writzl, K; Zorn, B; Peterlin, B | |
Title | Preliminary analysis of AZFb region duplication by quantitative real-time PCR | |
Type | članek | |
Source | Hum Reprod | |
Vol. and No. | Letnik 21, št. 3 | |
Publication year | 2006 | |
Volume | str. 753-4 | |
Language | eng | |
Abstract | Background: Deletions of the AZFb region on the long arm of the human Y chromosome cause male infertility. However, the reciprocal products of these deletion events, AZFb duplications, have not been reported to date. Furthermore, it is not known whether potential AZFb duplications represent a risk factor for spermatogenic failure. Methods: A total of 150 patients with male idiopathic subfertility (79 non-obstructive azoospermics and 71 oligozoospermics) and 150 fertile men were analysed for deletion/duplication of the sY125 locus and of the JARIDID gene using real-time PCR. Results: Three azoospermic men had deletion of the sY125 locus and of the JARIDID gene. No duplication was detected. Conclusions: In our limited sample, AZFb duplications do not appear to be associated with male infertility. | |
Descriptors | INFERTILITY, MALE Y CHROMOSOME OLIGOSPERMIA CHROMOSOME DELETION SEX CHROMOSOME ABNORMALITIES POLYMERASE CHAIN REACTION |