Author/Editor | Pristovšek, Primož; Simčič, Saša; Wraber, Branka; Urleb, Uroš | |
Title | Structure of a synthetic fragment of the lipopolysaccharide (LPS) binding protein when bound to LPS and design of a peptidic LPS inhibitor | |
Type | članek | |
Source | J Med Chem | |
Vol. and No. | Letnik 48 | |
Publication year | 2005 | |
Volume | str. 7911-4 | |
Language | eng | |
Abstract | Peptidic lipopolysaccharide (LPS) antagonists are the subject of intensive research. We report an NMR and modeling study of LBP-14 (RVQGRWKVRASFFK), a synthetic fragment of the LPS binding protein (LBP). In a mixture with LPS we observed the transferred nuclear Overhauser effect and determined the LPS-bound structure of LBP-14 that was used for docking calculations to LPS. The derived complex was used to design a peptide that displayed more than 50% increase in LPS inhibition in vitro. | |
Descriptors | LIPOPOLYSACCHARIDES ANTIBIOTICS CARRIER PROTEINS MEMBRANE GLYCOPROTEINS PEPTIDE FRAGMENTS MOLECULAR STRUCTURE TUMOR NECROSIS FACTOR LEUKOCYTES, MONONUCLEAR CELLS, CULTURED MAGNETIC RESONANCE IMAGING MODELS, MOLECULAR |