Author/Editor | Pećina-Šlaus, Nives; Kljaić, Milka; Nikuševa-Martič, Tamara | |
Title | Loss of heterozygosity of APC and CDH1 genes in laryngeal squamous cell carcinoma | |
Type | članek | |
Source | Pathol Res Pract | |
Vol. and No. | Letnik 201 | |
Publication year | 2005 | |
Volume | str. 557-63 | |
Language | eng | |
Abstract | The molecular mechanisms involved in the development and progression of laryngeal cancer, specifically squamous cell carcinoma, still need further investigation and elucidaition. Twenty-two laryngeal squamous cell carcinomas were analyzed in our study regarding genetic changes of two tumor suppressor genes: Adenomatous polyposis coli (APC) and E-cadherin (CDH1). APC gene instability was tested by polymerase chain reaction (PCR)/loss of heterozygosity (LOH) using the restriction fragment length polymorphism (RFLP) method. The samples were also screened for mutations using the heteroduplex method. E-cadherin gene was analyzed by PCR amplification of tetranucleotide marker (D16S7S2) linked to E-cadherin gene. The results of our analysis showed three samples with LOH of the APC gene out of 15 heterozygous patients (20%). Only one LOH of the CDHI gene (5.5%) out of 18 heterozygous patients was discovered. D16S752 marker did not reveal any replication error-positive samples. There were six samples showing heteroduplexes (33%) encompassed in APC's exon 11. Altogether, nine samples (41%) showed alterations of the APC gene. Our results suggest that alterations of APC gene may have a role in squamous cell carcinoma development. Detected LOH of the E-cadherin gene indicates that genetic changes of this gene are not very frequent, but that other components of the wnt signaling cascade may also be involved. | |
Descriptors | LARYNGEAL NEOPLASMS CARCINOMA, SQUAMOUS CELL GENES, APC CADHERINS DNA, NEOPLASM AGE FACTORS SEX FACTORS GENETIC MARKERS MICROSATELLITE REPEATS POLYMERASE CHAIN REACTION POLYMORPHISM, RESTRICTION FRAGMENT LENGTH HETEROZYGOTE |