Author/Editor		Hren, Matjaž; Baebler, Špela; Camloh, Marjana; Kovač, Maja; Ravnikar, Maja; Žel, Jana
Title		Yew (Taxus x media Rehd.) cell suspension cultures as a source of taxanes
Type		članek
Source		Acta Physiol Plant
Vol. and No.		Letnik 28, št. 1
Publication year		2006
Volume		str. 3-8
Language		eng
Abstract		Natural resources of paclitaxel, an effective anticancer compound, were threatened wilh extinction soon after the discovery of this valuable substance. Cell suspension cultures derived from different Taxus species have rapidly become an alternative source of paclitaxel and other taxanes. In this paper we provide some insight into cell growth characteristics in cell suspension culturc of Tuxus x media cv. Hicksii, with cmphasis on the effects of jasmonic acid (JA) on taxane production in cell lines with different initial taxane content. Additionally cell growth charactcristics of two cell lines was followed during cultivation of cell suspension culture of Tcrrus x rreedia cv. Hicksii. Packed cell volume (PCV) was shown to be a reliable and efficicnt alternative for measuring cell growth instead of fresh and dry weight. The initial total taxane content was screened in a number of cell lines, followed by observing the effece of JA on cell mass and total taxane production of selected lines. We showed a great variability in initial taxane content in different cell lines, which decreased during cell suspension maintenance. JA was shown to inhibit cell growth and increase total taxane production (14 to 106 fold).
Descriptors		TISSUE CULTURE PLANT GROWTH REGULATORS PACLITAXEL BIOMASS