Author/Editor		Poklar-Ulrih, Nataša; Skrt, Mihaela; Veranič, Peter; Galvani, Vesna; Vranac, Tanja; Čurin-Šerbec, Vladka
Title		Oligomeric forms of peptide fragment PrP(214-226) in solution are preferentially recognized by PrPSc-specific antibody
Type		članek
Source		Biochem Biophys Res Commun
Vol. and No.		Letnik 344
Publication year		2006
Volume		str. 1320-6
Language		eng
Abstract		A specific monoclonal antibody (mAb) V5B2 that discriminates between brain tissue of Creutzfeldt-Jakob disease patients and that from normal controls without proteinase K digestion has been prepared using a 13-residue synthetic peptide P1 from the primary structure of human PrP. In the light of the specific interaction between mAb V5B2 and the pathological isoform of PrP (PrP(Sc)), we investigated the solution behavior of antigen P1 and its interactions with mAb V5B2. Our results show that V5B2 recognizes epitope P1 in dimeric/oligomeric forms in solution and in the fibril-like aggregates, as well as in PrP(Sc) aggregates, and demonstrate that the specific epitope is present in all of these forms, but not in PrP(C).
Descriptors		ANTIBODIES, MONOCLONAL CREUTZFELDT-JAKOB SYNDROME PRIONS CIRCULAR DICHROISM PEPTIDE FRAGMENTS HYDROGEN-ION CONCENTRATION PROTEIN CONFORMATION TEMPERATURE